A Toolkit investigation typically comprises the following steps:
- propose a collagen-binding target (usually protein)
- provide proof that target binds collagens I, II or III, but not our control peptides
- bring target material to this lab and map onto Toolkits
- design and synthesise truncated and substituted THPs for "epitope-mapping"
- synthesise target-specific short THP for further studies.
We invite collaborators into the lab with their targets, to conserve Toolkit peptides that would be rapidly exhausted if shipped out in mg quantities to prospective users.
Steps 1 to 3 can be completed in a week or two, but 4 and 5 may take one month of synthetic activity per identified site.
Our general policy will be to provide access to the Toolkits upon request, subject to the availability of experimental time and providing there is no conflict of interest with other Toolkit investigations. The Advisory Board (Prof Flip de Groot, UMC Utrecht, Prof Hideaki Nagase, Kennedy Institute London, and Prof Richard Farndale, University of Cambridge) will consider requests for access on a monthly basis.
The Toolkit project is supported by a Biomedical Resource Grant from the Wellcome Trust, and to enable us to prolong the Resource, we will ask Toolkit users to contribute to the cost of replacing Toolkit peptides and of subsidiary syntheses and some consumables, as well as funding their own travel. Any joint IP (intellectual property) that arises will be developed by Cambridge Enterprise with the collaborator's technology transfer office.
The sequences of the peptides that comprise Collagen Toolkit II and Collagen Toolkit III are found via the top link on the left.